The Journal of Horticultural Science & Biotechnology
Vol. 63 No: 4
Seed Teguments as an Aid in Citrus Chemotaxonomy
Y. GOGORCENA and J.M. ORTIZ
Seed teguments from sour orange cultivars were used as the plant material for protein extraction and analysis. Soluble proteins separated by PAGE resulted in patterns that permitted distinction between cultivars. Peroxidases and esterases were found to be adequate for identification at the species level. Two cultivars, Amargo Clementino and Bergamoto segregated from the main cluster in the three-dimensional representation, their levels of dissimilarity being 1.40 and 2.47 respectively in the dendrogram obtained. Their assignment as hybrids or as species different from sour orange is discussed. Three bands with MW 45 000, 31 000 and 21 500 Daltons are tightly related to ‘Amargo Clementino’ and two bands (MW 50 000 and 35 000) to ‘Bergamoto’. The use of seed tegument proteins in the chemotaxonomy of citrus is suggested.
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