Vol. 69 No: 3

Title:
Recovery of Haploid and Diploid Plantlets from Anther Culture of Citrus Clementina Hort. ex Tan. and Citrus Reticulata Blanco

Authors:
M.A. GERMANÀ, YU YING WANG, M.G. BARBAGALLO, G. IANNOLINO and F.G. CRESCIMANNO

pp: 473-480

Abstract:
Research on androgenesis in two clementine cultivars (Nules and S.R.A. 63) and two mandarin cultivars (Avana and Tardivo di Ciaculli) was carried out with the aim of obtaining haploids. The anthers, collected at the uninucleated stage were cultured first on 11 media, differing either in the basic medium, the growth regulators, the carbon source and concentration, or the presence of activated charcoal. Calli, shoots, embryos and then plantlets were obtained. Significant differences were observed between the different cultural conditions and genotypes. Cytological observations on calli and plantlets from ‘Nules’ anthers revealed the haploid chromosome number, while mandarin calli and plantlets, and S.R.A. 63 calli had the diploid chromosome number. Electrophoretic analyses on calli and leaf tissues showed that ‘Nules’ had a homozygous genotype and confirmed that these tissues had developed from microspores.

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