Vol. 69 No: 3

Title:
An Improved Surface Disinfection Method for Shoot Explants from Iris Rhizomes Infected with Bacterial Soft Rot (Erwinia Carotovora subsp. Carotovora)

Authors:
F. BERGER, W.M. WAITES and C. LEIFERT

pp: 491-494

Abstract:
The standard method (protocol 1) for disinfecting shoot explants used for initiation of plant tissue cultures failed when Iris rhizomes were infected with bacterial soft rot (Erwinia carotovora subsp. carotovora). Two new disinfection protocols were therefore developed. They involved keeping stock plants without irrigation for two months prior to excision of explants; alcohol disinfection of the explants prior to excision from the rhizome; dipping of the explant in alcohol immediately after excision; soaking in 1% (v/v) sodium hypochlorite (protocol 2) or saturated calcium hypochlorite (protocol 3); and soaking in mercuric chloride. With protocols 2 and 3 up to 90% and 62% respectively of explants taken from infected Iris rhizomes showing soft-rot symptoms remained free of detectable microorganisms in vitro.

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