Vol. 69 No: 3

Title:
Freeze Preservation of Rose Pollen in Liquid Nitrogen: Feasibility, Viability and Fertility Status after Long-Term Storage

Authors:
P.E. RAJASEKHARAN and S. GANESHAN

pp: 565-570

Abstract:
The pollen cryobehaviour of four rose cultivars was studied. Pollen retained viability as indexed by in vitro germination after 48 h and one year (three cultivars) in liquid nitrogen. Cryostored pollen retained its ability to fertilize and produce seeds. The availability of cryostored pollen would enable rose breeders to pollinate at any time of the year at any location. Cryogenic preservation also offers the possibility of conserving the haploid gene pool in rose species through a pollen cryobank facility.

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