Vol. 69 No: 4

Title:
Temporary Modification of Adult Filbert Proliferation Capacity by Sequential Subcultures: Intensive Pruning as a Pre-Treatment for in vitro Reinvigoration

Authors:
C. DIAZ-SALA, M. REY and R. RODRIGUEZ

pp: 673-678

Abstract:
The improvement in shoot production of filbert (Corylus avellana L.) adult tissues by sequential in vitro subcultures and intensive pruning was studied. Improved and sustained production of adult shoots is possible by repeated subculturing in the presence of high cytokinin concentrations, which seem important for stabilizing the response and inducing a transient increase in proliferation. Intensive pruning increases proliferation in initial explants and facilitates the establishment and maintenance of culture lines. Rooting capacity is also enhanced by sequential in vitro subcultures, but stabilizing the response takes longer than with shoot proliferation. The increased capacity of adult tissues for shoot proliferation and rooting are similar to those obtained using juvenile plants as a source of explants, and could be explained as a reinvigoration induced by in vitro sequential subcultures.

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