The Journal of Horticultural Science & Biotechnology
Vol. 69 No: 6
In vitro Morphogenesis of Juvenile Annona Cherimola Mill. Bud Explants
C.L. ENCINA, A. BARCELÓ-MUÑOZ, A. HERRERO-CASTAÑO and F. PLIEGO-ALFARO
A micropropagation procedure for juvenile cherimoya (Annona cherimola Mill.) is described. Axillary shoot proliferation was obtained after culturing nodal sections with lateral buds in basal medium supplemented with either 0.66 µM BA or 1.36 µM zeatin. For root induction shoots were pre-incubated for 7 d in basal medium supplemented with 1 gl-1 activated charcoal, then cultured for 10 d (7 dark/3 light) on medium with 500 µM IBA, 58.4 mM sucrose, and 200 mg l-1 citric acid. Afterwards, shoots were transferred to the same medium without auxin and with the macroelements at half strength. Using this procedure, 95% of shoots rooted. The survival rate at the end of the acclimatization period was 70%.
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