Vol. 70 No: 1

Title:
Identification of Gerbera Cultivars Based on Differences in Phenolic Compounds in Ray Florets

Author:
G. BOOY

pp: 135-146

Abstract:
A method is described for the identification of gerbera cultivars using HPLC-analysis of phenolic compounds from ray florets. Anthocyanins and flavonoid copigments were analyzed in 65 genotypes. Different criteria for minimum peak area (pa_min = 50, 100 or 1000 mVs) and peak matching (maximum difference in peak area, pd_max = factor 2 or 10) were used. The discrimination capacity for anthocyanins ranged from 92.3% of all 2080 pairwise comparisons, using pa_min = 50 and pd_max = 2, to 70.5%, using pa_min = 1000, pd_max = 10. For flavonoid copigments, the discrimination capacity ranged from 98.9% (pa_min = 100, pd_max = 2) to 89.9% (pa_min = 100, pd_max = 10). By combining the anthocyanin data (with pa_min = 50) and the copigment data (with pa_min = 100), using pd_max = 2, a discrimination capacity of 99.5% was obtained. No relation was found between copigment composition and floret colour. For anthocyanins, the yellow and white genotypes formed one cluster. Generally, the reddish and pinkish/purplish genotypes were also clustered to a certain extent. Within these groups, however, no clear relation between floret colour and clustering was found, probably due to the interference of carotenoids.

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