Vol. 70 No: 1

Title:
Production of High-Frequency Embryogenic Calli from Integuments of Immature Seeds of Carica Papaya, L.

Authors:
S. MONMARSON, NICOLE MICHAUX-FERRIERE and C. TEISSON

pp: 57-64

Abstract:
Tissue culture conditions that enable production in Carica papaya L. of high frequency embryogenic calli on the internal integuments of immature seeds are defined: The explants have to be removed three or four months after anthesis and cultured on the three monthly successive media: M1-1 (2,4-D 9 µM), M2 (2,4-D 0.09 µM + KIN 1 µM), M3 (M2 + ABA 0.5 µM). A histocytological study during culture proved that friable calli capable of producing embryos were of maternal origin. This procedure thus permits the cloning of adult plants.

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