Vol. 70 No: 3

Title:
Long-Term Somatic Embryogenesis from Immature Olive Cotyledons

Authors:
ANNARITA LEVA, R. MULEO and RAFFAELLA PETRUCCELLI

pp: 417-422

Abstract:
A method for obtaining whole plants from immature cotyledons via somatic embryogenesis is described. Cultures were initiated by placing cotyledonary tissues at different developmental stages on SH medium supplemented with NAA and 2iP at different concentrations as an embryo induction medium (EIM). Proliferating masses of adventitious embryos were produced by cvs Frangivento, Picholine and Frantoio when transferred to the embryo expression medium (EEM) characterized by the exclusion of growth regulators. ‘Frangivento’ was maintained on EEM for three years without showing any loss of embryogenic potential while cvs Picholine and Frantoio retained their embryogenic capacity for only three subcultures. Optimum conversion into plantlets for ‘Frangivento’ was observed. Plants established in the greenhouse showed normal growth without change in performance. The protocol described makes it possible to maintain long-term embryogenic capacity; this may be particularly advantageous for propagation and biotechnology applications.

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