Vol. 70 No: 5

Title:
In Vitro CO₂ Enrichment of CAM Orchid Plantlets

Authors:
C.S. HEW, S.E. HIN, J.W.H. YONG, S.S. GOUK and M. TANAKA

pp: 721-736

Abstract:
Increased growth of an in vitro-propagated CAM orchid hybrid Mokara ‘White’ was obtained using a novel method of CO₂ enrichment in an optimized photoautotrophic open system compared with the conventional closed system of culture. The optimization process for the open system involved the manipulation of external CO₂ concentrations (0.03%, 1% and 10%), sucrose requirements, light intensities (80 and 200 µmol m^-2 s^-1) and the venting of headspace ethylene from the culture vessels. The physiological basis for increased growth in these CAM orchid plantlets after three months was attributed to the direct effects of elevated CO₂ resulting in higher CAM activity for the plantlets and to the elevated CO₂ present in the system which might interact with the ethylene present thereby reducing the inhibition of growth of plantlets due to ethylene.

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