Vol. 70 No: 6

Title:
The Development of Putative Adventitious Shoots from a Chimeral Thornless Rose (Rosa Multiflora Thunb. ex J. Murr.) in vitro

Authors:
A. ROSU, R.M. SKIRVIN, A. BEIN, M.A. NORTON, M. KUSHAD and A.G. OTTERBACHER

pp: 901-908

Abstract:
A method has been developed for producing putative adventitious shoots from proliferating shoots of a chimeral Rosa multiflora rootstock. Proliferating cultures were established from greenhouse-grown axillary buds on Skirvin and Chu's (1979) modification of Murashige and Skoog (1962) medium supplemented with 6-benzylamino purine (BA, 2 mg l^-1) and naphthaleneacetic acid (NAA, 0.1 mg l^-1). The buds typically expanded to produce a single shoot that soon yellowed and senesced. However, when buds were transferred to the same medium with BA replaced by gibberellic acid (GA₃, 0.5 to 1.0 mg l^-1) and silver riitrate (3.4 mg l^-1), prior to the onset of senescence, the shoots continued to expand and proliferate following subculture at 3–4 week intervals. Shoots harvested from this medium were moved to the same medium supplemented with various levels of thidiazuron (TDZ). Those subcultured on medium with 1 µM TDZ developed compact nodular callus that later, after one or two subcultures onto the same medium, formed putative adventitious shoots. About half of these shoots rooted on MS medium supplemented with three auxins [(NAA, 0.5 mg l^-1); indoleacetic acid (IAA, 1 mg l^-1), and indolebutyric acid (IBA, 0.5 mg l^-1)], GA₃ (0.5 mg l^-1), silver nitrate (3.4 mg l^-1), activated charcoal (200 mg l^-1) and sucrose (40 g l^-1). A sample (119) of these plants were screened for their thorny or thornless condition: some parental stems had a few recurved thorns; petioles had small lignified hairs. All regenerants had thornless stems; but they varied in degree of petiole prickliness. This suggests that we have separated a pure thornless form from the parental clone. In addition, there is enough variation among regenerants to suggest that somaclonal variation has appeared.

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