Vol. 71 No: 1

Title:
Influence of Growth Regulators and Carbon Sources on Callus Induction, Growth and Morphogenesis from Leaf Tissues of Peach (Prunus Persica L. Batsch)

Authors:
VERONIQUE DECLERCK and S.S. KORBAN

pp: 49-56

Abstract:
Leaf segments of Prunus persica (L.) Batsch ‘Elberta Queen’ were collected from in vitro-grown proliferating shoots and cultured onto a basal medium containing half-strength Murashige and Skoog (MS) salts, Staba vitamins, 30 g l^-1 sucrose and 6.5 g l^-1 Difco-bacto agar. The influences of six growth regulators supplemented at three levels (5, 10 and 15 µM) on callus induction were investigated under light conditions (16 h photoperiods). For all growth regulator treatments, caulogenesis was observed along cut margins of leaf explants. Among cytokinins tested, thidiazuron (TDZ) induced compact green calli, 6-benzyladenine (BA) and zeatin induced small calli, and kinetin failed to induce callus. For auxin treatments, both dicamba and 2,4-D induced friable white to yellowish calli. In another experiment, leaf explants collected from greenhouse-grown ‘Bellaire’ and in vitro-grown ‘Elberta Queen’ were cultured onto the basal medium supplemented with a wide range of TDZ concentrations (3 to 23 µM) Caulogenesis was optimum for both genotypes at TDZ concentrations of 8 to 13 µM, while higher TDZ levels resulted in necrosis of leaf explants. In a third experiment, the influence of glucose, fructose, and sucrose on caulogenesis was investigated. Leaf explants grown on media containing glucose developed a higher frequency of green and compact calli than those grown on either fructose or sucrose.

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