Vol. 71 No: 1

Title:
Genetic Deviation Initiated by Adventitious Shoot Regeneration from Tissue Cultured Red Raspberry

Authors:
A.-S. HOEPFNER, R. NESTBY and H. NYBOM

pp: 71-80

Abstract:
Adventitious shoots were regenerated, using leaf segments from in vitro cultures of a Norwegian red raspberry (Rubus idaeus L.) selection, N71-B3 (‘Distad’ × ‘Viking’). 1H-indole-3-butanoic acid (IBA) in combination with either N-(phenylmethyl-1H-purin-6-amine (BA) or N-phenyl-N'-1,2,3-thiadiazol-5-ylurea(thidiazuron or TDZ), were added to MS media. Media were evaluated for regeneration rates, rooting ability of regenerants, genetic stability of phenotype and DNA fragment profiles. There was a positive association between low growth regulator concentration as compared with high regeneration and high rooting percentages as well as low off-type production. BA gave a higher regeneration and rooting percentage than TDZ, while the development of off-types was at a comparable level at similar cytokinin concentrations (BA_high vs. TDZ_low). When DNA fingerprinting was used to compare divergent phenotypes with control plants, no variation in fragment patterns could be detected by hybridization of Hae III digested DNA samples to the M13 probe or the (TG)n probe.

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