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The Journal of Horticultural Science & Biotechnology

Vol. 71 No: 2

Title:
Chlorophyll, Ethylene and Biomass Determination in Prunus Avium cv. Victoria Cultivated in Vitro under Different Atmospheric Conditions

Author:
B. RIGHETTI

pp: 249-256

Abstract:
Physiological, morphological and growth characteristics were evaluated in Prunus avium cv. Victoria, grown under photomixotrophic conditions, at three different atmospheric compositions over a 30 d culture period. In a CO2-free environment a decrease in chlorophyll was observed during the first half of the culture period followed by regreening. Very low ethylene, and lower fresh weight (FW%) and dry-matter percentages (DM%) were also observed. In cultures grown at 0.034% CO2-21% O2, chlorophyll rapidly increased during the first 15–18 d, followed by a decrease. In these conditions shoots were well developed and lignified with large leaves and no hyperhydricity. Higher fresh-weight and dry-matter percentages, along with higher ethylene levels, were observed. In closed vessels, shoots were irregularly shaped, with curling leaves and hyperhydricity, and a moderate chlorophyll content with a decreasing trend after 15–18 d of culture. Under these conditions the lowest dry-matter percent production and the highest ethylene synthesis were also observed. The relationship between CO2, chlorophyll and ethylene is discussed.

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