Vol. 71 No: 2

Title:
Effects of Gamma Radiation on in vitro Plantlets of Alpinia Purpurata

Authors:
L. FEREOL, S. LOUIS and L. LUCE

pp: 243-248

Abstract:
Two cultivars of Alpinia purpurata were propagated by in vitro culture to provide numerous convenient plantlets that were exposed to 0, 15, 30, or 45 Gy of gamma rays to study their radiosensitivity. The plants of the C1 and C2 clonal generations showed decreases in plant survival rate, fresh weight gain and micropropagation rate with increasing doses of gamma radiation. On the basis of survival, LD 50 was approximately 30 Gy. The 45 Gy dose drastically affects the different responses studied. It proved lethal or induced dwarf plants. Some plantlets, produced in vitro from treated plants, showed morphological abnormalities of leaves or of whole plants. This technique has potential for increasing the genetic variation in Alpinia purpurata.

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