Vol. 72 No: 3

Title:
Micropropagation of Young and Adult Plants of Cypress (Cupressus Sempervirens L.)

Authors:
M. CAPUANA and R. GIANNINI

pp: 453-460

Abstract:
A method is described to induce the axillary bud proliferation, elongation and rooting in explants of cypress of different ages (i.e. five week old seedlings, 15 year old and more than 150 year old trees). Bud induction was realized on SH medium containing 5 µM BA and 0.1 µM NAA and the highest number of shoots per explant (4.5) was obtained for the material collected from the five week old seedlings. The shoot elongation was realized by adding 0.1 g l^-1 to the plant growth regulator-free medium. For rooting, IBA or IAA treatments were effective, particularly those consisting of 7 d culture on medium containing 0.1 mM IBA. The age of the trees from which the initial explants were collected strongly affected all phases of the in vitro multiplication; proliferation rate, successive growth and rooting were highest for the explants collected from the youngest plants. Rooting resulted the most problematic step of the multiplication cycle: although the explants collected from young plants rooted easily, the material proliferated from aged trees gave very low percentages of rooting.

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