Vol. 72 No: 5

Title:
Effects of Explant Length and Diameter on in vitro Shoot Growth and Proliferation Rate of Miniature Roses

Authors:
H. SALEHI and M. KHOSH-KHUI

pp: 673-676

Abstract:
The influence of different explant sizes on in vitro shoot growth and development were studied in the three miniature rose cultivars Little Buckaroo, Baby Masquerade and Sourati. Single-node explants with combinations of 4.0–5.0 or 9.0–10.0 mm lengths and 2.0–2.5 or 3.0–3.5 mm diameters were cultured on establishment media. Shoot length and proliferation rate were measured one and four weeks after culture, respectively. In general, the best shoot development and proliferation obtained by explants with the greatest length and diameter. In ‘Little Buckaroo’, a positive correlation was observed between shoot length after one week and proliferation after four weeks.

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