Vol. 73 No: 2

Title:
In Vitro Flowering on Long-Term Subcultured Pear Shoots

Authors:
H. HARADA and Y. MURAI

pp: 225-228

Abstract:
In vitro flowering was induced from long-term (for six years) subcultured shoots of pear (Pyrus communis L.). Shoots were subcultured every 30 d on WP medium (BA 1 mg l^-1, sucrose 3%, agar 0.8%) and maintained for six years. Flower induction occurred when shoots were cultured on MS medium supplemented with CPPU at 1 or 5 mg l^-1 and incubated at 15°C for 15 or 30 d. Shoots cultured on WP medium supplemented with CPPU did not flower. Shoots cultured on MS or WP medium supplemented with CPPU and maintained at 26°C also did not flower. Flowering was observed soon after the treated shoots were transferred to MS medium supplemented with BA 1 mg l^-1 and incubated at 26°C. Flowering percentages were 4 to 12%. Each flower consisted of five sepals, five white petals, several stamens, and one pistil. Pollen grains were formed, but development of the ovary and ovules was incomplete.

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