Vol. 73 No: 3

Title:
Effect of Different Treatments on the Conversion of M.26 Apple Rootstock Synthetic Seeds Obtained from Encapsulated Apical and Axillary Micropropagated Buds

Authors:
G. CAPUANO, E. PICCIONI and A. STANDARDI

pp: 299-306

Abstract:
Encapsulated buds excised from micropropagated shoots of the clonal apple rootstock M.26 (Malus pumila Mill.) can be considered an interesting example of non-embryogenic synthetic seeds. Two experiments were carried out to evaluate the different aptitude of apical and axillary buds towards encapsulation and synthetic seed production, and to find the best treatments to enhance conversion rates. Different behaviour between axillary and apical buds was observed. Synseeds from apical buds reached conversion rates of 85%, against a maximum of 25% obtained with the encapsulated axillary buds. A root induction treatment with 24.6 µM IBA followed by a root initiation culture of 3 to 6 d prior to encapsulation with a nutrient-enriched artificial endosperm proved to be the best protocol for enhancing rooting of the encapsulated explants. A cold treatment of 60 d was able to reduce formation of undesired callus on the explants, which was negatively correlated with rooting.

Full text: JHSB Subscribers     ISHS members & other users
(PDF 1333290 bytes)

Translate:

Go back to previous page