Vol. 73 No: 3

Title:
Phylogenetic Relationships of the Common Durian (Durio Zibethinus Murray) to Other Edible Fruited Durio spp. by RFLP Analysis of an Amplified Region of CpDNA

Authors:
SHINYA KANZAKI, KEIZO YONEMORI, AKIRA SUGIURA and SURANANT SUBHADRABANDHU

pp: 317-322

Abstract:
Phylogenetic relationships among seven species of the genus Durio, of which four produce edible fruits including the common durian (D. zibethinus), were investigated from RFLP analysis of PCR products from a variable region of cpDNA. The target region of cpDNA was reliably amplified from all Durio species tested, using total DNAs extracted by a modified CTAB method of Doyle and Doyle (1987) followed by an additional purification step. When restriction fragment polymorphisms of amplified products were examined with 19 restriction endonucleases (AseI, BamHI, BfaI, DraI, EcoRI, EcoRV , HindIII, KpnI, MspI, RsaI, ScaI, ScrFI, SalI, SmaI, SspI, StyI, TaqI, XbaI, XhoI), RFLPs were observed with 14 endonucleases. However, since all Durio species tested had length mutation on this amplified region of cpDNA, the most parts of diversities from 14 endonucleases were caused by the length mutations among species. Considering length mutation among species, a total of seven site mutations were detected as well as 12 length mutations, based on D. zibethinus. When unordered parsimony analysis was conducted using these data, the cultivated durian (D. zibethinus) did not show any close relationships to other three species producing edible fruits. D. zibethinus formed a cluster with wild species, D. griffithii, and other three species made two clusters with D. lowianus or D. mansoni. Our results indicated an independent evolutionary process among four edible species of the genus Durio.

Full text: JHSB Subscribers     ISHS members & other users
(PDF 2015525 bytes)

Translate:

Go back to previous page