Vol. 73 No: 5

Title:
Genetic Relationships of Viola Species Evaluated by Random Amplified Polymorphic DNA Analysis

Authors:
M.K. KO, J. YANG, Y.H. JIN, C.H. LEE and B.J. OH

pp: 601-605

Abstract:
The genetic relationships of Viola species in the subsection Patellares were analysed using the random amplified polymorphic DNA (RAPD) method. Viola species from the series Chinensis and Pinnatae, and five hybrid lines formed a cluster which divided into three subgroups. Viola species in the series Variegatae and a hybrid line were clustered into a second group. The clustered (sub)groups from RAPD analysis are in agreement with the conventional taxonomy based on morphological characters. Therefore, RAPD analysis can be used for providing an alternative classification system to identify genotypes and morphological characters of Viola species. However, although V. chaerophylloides, and V. albida and V. albida var. takahasii were classified into different series, Pinnatae and Chinensis, by morphological characters, they were clustered together with a high similarity coefficient by RAPD analysis. Therefore, we suggest that these species should be classified into Pinnatae. Also, RAPD analysis was successfully applied to deduce putative parents for six unknown hybrid lines.

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