Vol. 73 No: 6

Title:
Ventilation of Culture vessels. I.Increased Growth in vitro and Survival ex vitro of Delphinium

Authors:
K.P. MURPHY, J.M. SANTAMARIA, W.J. DAVIES and P.J. LUMSDEN

pp: 725-729

Abstract:
Inclusion of small circular apertures covered with filters in the sides of plastic culture vessels led to a small but significant increase in the multiplication rate of Delphinium cultured in vitro and greater survival following transfer ex vitro. Filters had no effect on the multiplication rate and survival of Hosta. In vessels with apertures there was a large increase in the rate of water loss, but relative humidity was greater than 95% in both intact vessels and in vessels with filters. It is suggested that in vessels with apertures there was an increase in the flow of water vapour from the vessel atmosphere to the external atmosphere, due to a reduced diffusive resistance. The improved performance of Delphinium plants could have resulted from an increase in transpiration and movement of water (and therefore some nutrients) through the plants.

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