Vol. 74 No: 5

Title:
Feasibility and anatomical development of an in vitro olive cleft-graft

Authors:
A. TRONCOSO, JUANA LINAN, M. CANTOS, M.M. ACEBEDO and HAVA F. RAPOPORT

pp: 584-587

Abstract:
The feasibility and anatomical development of an in vitro olive cleft-graft method were studied. Grafting survival after 60 d in vitro was 85% which then dropped slightly to 67% after hardening. Three days after grafting, callus formation was observed along the cut surfaces of the rootstock and scion, after 6 d the first healing cellular unions were observed, and by 12 d after grafting a strong union developed. The first cellular differentiation to form vascular tissues was observed 12 d after grafting and continued rapidly until a total connection was reached 10 d later. Thus a complete graft union of the in vitro olive cleft-grafts was achieved in three weeks, and vigorous plants were established after 60 d of in vitro culture followed by 10 d of hardening. The in vitro olive cleft graft is suggested as an effective and useful method for germplasm multiplication.

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