Vol. 75 No: 3

Title:
In vitro evaluation of apricot (Prunus armeniaca L.) resistance to plum pox potyvirus

Authors:
P. MARTÍNEZ-GÓMEZ and F. DICENTA

pp: 259-261

Abstract:
An in vitro method for evaluating the resistance of apricot to plum pox potyvirus (sharka) was established. Three assays were performed: A) Micro-grafting the apricot to be evaluated onto a GF305 seedling that had been germinated in vitro and inoculated with PPV by micro-grafting. B) Micro-grafting the apricot onto a shoot of GF305 previously inoculated in vivo and afterwards grown in vitro. C) Inoculation of an apricot shoot with an infected chip-bud of a diseased GF305 grown in vitro. In vitro evaluation showed a high degree of correspondence with evaluations in vivo, the first method (A) being the most effective for evaluating resistance. In vitro evaluation, therefore, could be a suitable method for determining the resistance to PPV of seedlings obtained in a breeding programme, either as an alternative, or, preferably, as a complement to traditional in vivo evaluation.

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