Vol. 75 No: 4

Title:
Plant regeneration from anther derived callus cultures of ginger (Zingiber officinale Rosc.)

Authors:
K. SAMSUDEEN, K. NIRMAL BABU, MINOO DIVAKARAN and P.N. RAVINDRAN

pp: 447-450

Abstract:
Ginger anthers collected at the uninucleate microspore stage were subjected to a cold treatment (0°) for 7 d and induced to develop profuse callus on MS medium supplemented with 2-3 mg l^-1 2, 4-D. Plantlets could be regenerated from these calli on MS medium supplemented with 5-10 mg l^-1 BAP and 0.2 mg l^-1 2,4-D. The regenerated plantlets could be established in soil with 85% success, when they were planted in potting mixture of garden soil, sand and vermiculite in equal proportions and kept in a humid chamber initially for 22-30 d. This is the first report of successful regeneration of plants from ginger anther cultures and the protocol can be used for possible development of androgenic haploids and dihaploids in ginger.

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