Vol. 76 No: 1

Title:
Micropropogation of Plumago zeylanica L. by encapsulated nodal explants

Authors:
G.R. ROUT, G. DAS, S. SAMANTARAY and P. DAS

pp: 24-29

Abstract:
Nodal explants excised from aseptic shoot cultures of Plumbago zeylanica were encapsulated in a sodium alginate matrix under aseptic conditions. The development of axillary shoots depended on the components of the gel matrix. The gel matrix consisting of MS basal salts supplemented with 1.0 mg l ±1 BA, 0.01 mg l ±1 IAA resulted in the best morphogenetic response, compared with the matrix without growth regulator. Increase in the concentration of IAA (0.1 mg l ±1) reduced the rate of bud sprout and shoot multiplication. The alginate beads cultured on semi-solid culture medium having BA + IAA resulted in a greater morphogenetic response than BA + NAA. The encapsulated beads exposed to 50 μM CaCl₂. H₂O for 15 min significantly increased bud sprout, shoot multiplication and rooting. Encapsulated beads exposed to 50 μM CaCl₂. H₂O for more than 25 min reduced the morphogenetic response. The encapsulated derived plants were successfully grown-on in soil.

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