Vol. 76 No: 2

Title:
Expression of dehydrin-like genes in response to chilling in leaves of blackcurrant, Ribes nigrum L.

Authors:
P.G. LANHAM, R.G.N KEMP, H. JONES and R.M. BRENNAN

pp: 201-207

Abstract:
We report the isolation of dehydrin-like genes (dhns) from cold-acclimated leaves of blackcurrant plants using RT-PCR with a guessmer primer based on known dehydrin DNA sequences. Several Dhn-like c-DNAs from Ribes nigrum were isolated and one (b8) with an internal lysine-rich coding sequence (K segment) was chosen for further characterization. Northern analysis conclusively demonstrated that b8 was chill-induced in vegetative tissues of R. nigrum (no transcripts could be detected at normal growth temperature). The cold induction of dhns was confirmed at the protein level using a polyclonal antibody immunoreactive to the K segments in DHNs.

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