Vol. 76 No: 2

Title:
A functional S-allele, 'Sg', in the wild apple possessing a single amino acid, S-RNase 'Sg'-RNase', different from 'Sg-RNase' in Malus × domestica cultivars

Authors:
SHOGO MATSUMOTO, KENTARO KITAHARA, HIROMITSU KOMATSU and JUNICHI SOEJIMA

pp: 163-166

Abstract:
S3-, S5-, S9- (=Sc-), Sf-, Sg- and Sh (=S24)-alleles in the wild apple were deduced by using S-allele-specific- polymerase-chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analyses. Within the alleles, the deduced amino acid sequence of Sg'-RNase in Sg-allele of Malus transitoria differed by one amino acid from that of Sg-RNase in Sg-allele of M. × domestica 'Indo'. From the cross pollination analysis of M. transitoria and M. × domestica 'Indo', and S-allele-specific-PCR-RFLP analyses of their progenies, it was indicated that the pistil Sg'- RNase in M. transitoria rejected the pollen of Sg-allele from 'Indo'. A difference of one amino acid within the RHV region produced by natural mutation was not enough to generate a new S-allele. This is the first report in the literature concerning S-allele diversification in the apple.

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