Vol. 76 No: 4

Title:
In vitro plant regeneration and gene transfer in the wild tomato Lycopersicon cheesmanii

Authors:
I. ARRILLAGA, C. GISBERT, E. SALES, L. ROIG and V. MORENO

pp: 413-418

Abstract:
This paper reports on protocols for in vitro plant regeneration, gene transfer and on shoot salt tolerance from two accessions of L. cheesmanii (Riley). Shoot organogenesis was achieved on media with zeatin or BA/IAA combinations. The two accessions tested, LA1401 and LA530, showed differences in both their aptitude to regenerate and their salt tolerance in vitro. The accession LA530 had a higher organogenic potential than LA1401 (97% vs. 80% of organogenic explants). In contrast, none of the LA530 isolated shoots rooted when cultured in full strength MS- modified medium supplemented with 200 mM NaCl while 93% of shoots from LA1401 formed roots. Finally, Agrobacterium-mediated transformation was used to introduce the nptII and uidA marker genes into the salt tolerant accession LA1401. Eight to nine per cent of the cotyledon explants produced transgenic plants. After self-pollination, the inheritance analysis showed a Mendelian segregation of both transgenes. The collection of transgenic plants will be an useful tool in experiments of asymmetric somatic hybridization with L. esculentum.

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