Vol. 76 No: 5

Title:
cDNA cloning of NAD-dependent sorbitol dehydrogenase from peach fruit and its expression during fruit development

Authors:
K. YAMADA, N. NIWA, K. SHIRATAKE and S. YAMAKI

pp: 581-587

Abstract:
Full length cDNA for NAD-dependent sorbitol dehydrogenase (NAD-SDH; EC 1.1.1.14) was cloned from peach fruit by reverse transcription polymerase chain reaction (RT-PCR) and 3'/5' rapid amplification of cDNA ends (RACE). The full-length cDNA consisted of 1457 bp and contained an open reading frame of 1101 bp capable of encoding a protein of 367 amino acids. The deduced amino acid sequence revealed about 77% identity to apple NAD-SDH. Peach NAD-SDH activity based on FW was very strong in immature fruit and declined temporarily, then increased again with fruit maturation. The activity based on the whole fruit showed a peak at 54 and 69 d after flowering (DAF), declined temporarily and increased greatly with fruit maturation. The seasonal change of NAD-dependent sorbitol dehydrogenase (NAD-SDH) protein levels based on fresh weight (FW) corresponded to that of the activity based on FW. The typical post translational modification of this enzyme was not indicated. On the other hand, the seasonal change of NAD-SDH transcript levels did not correspond to that of activity based on FW, but roughly corresponded to that based on the whole fruit. From the above results, at least we suggest that NAD-SDH plays an important role in sorbitol metabolism during peach fruit development, and that the activity of NAD-SDH enhanced by fruit maturation is regulated by the transcription of the gene.

Full text: JHSB Subscribers     ISHS members & other users
(PDF 546816 bytes)

Go back to previous page