Vol. 76 No: 6

Title:
Molecular typing of Cereus peruvianus clones and their genetic relationships with other Cereus species evaluated by RAPD analysis

Authors:
F. GUTMAN, D. BAR-ZVI, A. NERD and Y. MIZRAHI

pp: 709-713

Abstract:
RAPD analysis was used to fingerprint 29 genotypes of Cereus species and examine their genetic diversity. Some modifications were made to the DNA extraction procedure with a view to assuring higher DNA yield and purity. A dissimilarity matrix and dendrogram were constructed to help visualize genetic relationships between different genotypes. The results show that C. peruvianus has only a limited genetic base and that further improvement of this crop may require the introduction of additional germplasm into breeding programmes. RAPD markers were shown to be a valuable tool for clone identification in Cereus species and for determining genetic relationships.

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