Vol. 76 No: 6

Title:
Integration of the rolA gene into the genome of the vigorous apple rootstock A2 reduced plant height and shortened internodes

Authors:
LI-HUA ZHU, ANNELIE AHLMAN, XUE-YUAN LI and MARGARETA WELANDER

pp: 758-763

Abstract:
The aim of this work was to dwarf the vigorous apple rootstock A2 by insertion of the rolA gene. To optimize conditions for a successful transformation, regeneration tests were carried out. The use of sucrose in the regeneration medium gave higher regeneration frequency than sorbitol in some cases and the shoot number per regenerated leaf was higher at 10 µM TDZ compared with 2.5 µM TDZ on the sucrose medium. Two transgenic clones, verified by PCR and Southern analysis, have been obtained on the sucrose medium together with 2.5 µM TDZ and 1.0 or 2.5 %micro;M NAA and wounding by forceps. The two clones, named LA1 and LA2, contained both the rolA and nptII genes. The results of in vitro rooting showed that LA1 had a lower rooting percentage and a reduced root number per rooted shoot than the untransformed control shoots and the clone LA2 on the rooting medium containing 5 µM IBA. Growth analysis revealed that both transgenic clones had a reduced plant height and a shortened internode length compared with the control plants. However, the node number and the stem diameter were significantly larger for clone LA1 than clone LA2 and the control plants.

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