Vol. 77 No: 3

Title:
Fate and transformations of ¹⁵N labelled nitrogen applied in spring to Citrus trees

Authors:
B. SERRANO, J. GOMEZ-APARISI and J.I. HORMAZA

pp: 368-372

Abstract:
Twenty five genotypes useful as rootstocks for different Prunus species have been screened with SSR primers developed in peach, to identify and characterize the genotypes and to study the genetic similarities among them. Eighteen primer pairs that resulted in repeatable easily scorable amplification patterns were sufficient to identify unequivocally all the genotypes studied. Those primer pairs produced a total of 95 alleles with a mean value of 5.3 alleles per locus. The results obtained show the usefulness of cross-species transportability of microsatellite sequences. Unweighted Pair Group Method with Arithmetic averages (UPGMA) cluster analysis of the similarity data grouped the genotypes studied according to their pedigree information separating the genotypes with almond and/or peach background from the rest of the species and interspecific hybrids included in the analysis.

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