Vol. 77 No: 5

Title:
Identification of RAPD and SCAR markers linked to the flesh adhesion gene F in peach (Prunus persica (L.) Batsch)

Authors:
J.H. JUN, K.H. CHUNG, S.B. JEONG and H.J. LEE

pp: 598-603

Abstract:
Flesh adhesion to the stone in peach (Prunus persica (L.) Batsch) fruits can be categorized into three groups: freestone, semi-clingstone and clingstone. Flesh adhesion is known to be determined by a single gene pair F/f with the freestone dominant to clingstone. Bulked segregant analysis and randomly amplified polymorphic DNA (RAPD) analysis were performed to detect markers linked to the F/f gene using an F₁ progeny of clingstone ´Yumyeong` (ff) × freestone ´Baekhyang` (Ff). Four dominant coupling-phase RAPD markers linked to the gene, designated as OPB05₁₃₀₀, OPD20₅₈₀, OP107₁₀₅₀ and UBC439₁₄₀₀ were identified from 578 10-mer primers examined. The cosegregation of the four markers and the F/f gene was verified using the 95 individuals of the F₁ progeny of ´Yumyeong` × ´Baekhyang`. The RAPD markers OPB05₁₃₀₀, OP107₁₀₅₀ and UBC439₁₄₀₀ were found to be located at 2.2, 4.4 and 1.1 cM, respectively, on one side of the F/f locus and the marker OPD20₅₈₀ at 11.8 cM on the other side. Two RAPD markers OPBO5₁₃₀₀ and UBC439₁₄₀₀ were developed into dominant sequence characterized amplified region (SCAR) markers. The SCAR markers were found to be adequate to identify the F/f gene in segregating progenies and commercial cultivars. These markers can reliably be used in the marker-assisted selection of peach cross seedlings at an early developmental stage of the trees.

Full text: JHSB Subscribers     ISHS members & other users
(PDF 412601 bytes)

Translate:

Go back to previous page