The Journal of Horticultural Science & Biotechnology
Vol. 79 No: 3
Callus induction from protocorm-like body segments and plant regeneration in Cymbidium (Orchidaceae)
L.V.T HUAN and M TANAKA
Embryogenic callus induction and plant regeneration in Cymbidium Great Flower ´Rainbow Drop` were examined. Longitudinally bisected segments of protocorm-like bodies (PLBs) formed calli within about one month on modified Vacin and Went medium (1949) supplemented with 1-naphthaleneacetic acid (NAA) or 2,4- dichlorophenoxyacetic acid (2,4-D) alone or in combination with N-phenyl-N`-l, 2, 3-thiadiazol-5-yl urea (TDZ).The combination of 0.5 mg l-1 NAA and 0.1 mg l-1 TDZ was found to be the most effective for callus induction.The calli grew well when subcultured every four weeks on the same medium. The calli easily formed PLBs after being transferred to media without plant growthregulators. Healthy plantlets without any phenotypic abnormality were obtained from callus-derived PLBs.
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