Vol. 79 No: 4

Title:
An RAPD marker linked to the pollen sterility gene ps in peach (Prunus persica)

Authors:
J. H. JUN, K. H. CHUNG, S. B. JEONG and H. J. LEE

pp: 587-590

Abstract:
Pollen sterility in peach (Prunus persica [L.] Batsch) is known to be determined by a single gene pair Ps/ps with pollen sterility recessive to pollen fertility. Crossing of pollen-fertile ´Yumyeong` with pollen-fertile ´Baekhyang` gave both pollen-fertile and pollen-sterile F1 seedlings, in a ratio of approximately 3:1, indicating that both parents are heterozygous Psps. Bulked segregant analysis and randomly amplified polymorphic DNA (RAPD) analysis of this progeny were performed to detect markers likely to be linked to the Ps/ps gene. One RAPD marker coupled to the Ps allele, designated as UBC4052300, was identified from 578 10-mer primers examined. The co-segregation of the marker and the Ps/ps gene was verified in the 115 individuals of the full progeny and the recombination frequency between the marker and the Ps/ps locus was 4.3&percent;.The marker was found to be generally adequate to identify the Ps allele in another segregating progeny and commercial cultivars. This marker should be useful for the marker-assisted selection of peach seedlings soon after germination.

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