Vol. 79 No: 4

Title:
Enhanced in vitro production of colchicine in Gloriosa superba L. - an emerging industrial medicinal crop in South India

Authors:
G. SIVAKUMAR, K.V. KRISHNAMURTHY, E. J. HAHN and K.Y. PAEK

pp: 602-605

Abstract:
In vitro supply of exogenous precursors for the biosynthesis of colchicine using B₅ medium from Gloriosa superba L. calluses is reported. Determination of colchicine in callus samples has been established using high-performance liquid chromatography combined with mass spectrometry. The maximum amount of colchicine i.e. 9.0 mg g^-1 DW was detected in the medium fed with 30 μM tyrosine. Under this condition, activity of tyrosine ammonia lyase (TAL) was higher than that of phenylalanine ammonia lyase (PAL) and a low frequency of tracheary elements was formed.

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