Vol. 79 No: 5

Title:
Cloning of apricot RGAs (Resistance Gene Analogs) and development of molecular markers associated with Sharka (PPV) resistance

Authors:
L. DONDINI, F. COSTA, G. TATARANNI, S. TARTARINI and S. SANSAVINI

pp: 729-734

Abstract:
Plant disease-resistance genes show highly conserved regions corresponding to characteristic amino acid domains. These conservative structures make it possible to isolate resistance gene analogs (RGAs) by PCR using degenerate or heterologous primers. Putative apricot ('Stark Early Orange') RGAs were amplified, cloned and characterized using endonucleases; several divergent cloned-PCR products have been sequenced.The gene bank database screening showed a certain degree of homology with known resistance genes or RGA sequences. Specific primers designed on the basis of the hyper-variable regions of the sequences identified have been used to identify molecular markers for Sharka resistance. After screening of a panel of apricot genotypes (six resistant, ten susceptible and one tolerant), one primer pair (SEOBT101) appears to be associated with Sharka resistance.

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