Vol. 79 No: 5

Title:
Responses of in vitro cultured kiwifruit shoots to treatments with green amaranth aqueous extracts

Authors:
G. MARINO, M. HERNANDEZ, A. LUCCHI and A. ROMBOLA

pp: 759-763

Abstract:
Because of environmental hazards related to the use of synthetic Fe-chelates in Fe-chlorosis control of plants, and with the purpose of using plant extract treatments as an alternative strategy, the effect of Amaranthus retroflexus L extracts on Actinidia deliciosa Liang et Ferguson shoot cultures was investigated, and the possibility of using in vitro cultures for rapid evaluation of the effect of such extracts on growth and Fe-chlorosis control in fruit plants is discussed. The extract was obtained by macerating for a week 100 g plant fresh weight in 1 litre of distilled water, filter-sterilizing and adjusting the pH to 5.65; the extract was added at 0 (control), 20, 2, 0.2 and 0.02 ml to 100 ml of previously autoclavesterilized modified Murashige and Skoog, MS, medium (PM), and salt-deficient PM/3 (third MS salt strength) and rooting RM (lacking growth regulators, half MS salt strength) media. The addition of 20 ml water extract to 100 ml PM/3 strongly reduced shoot and basal callus development, while 0.02 ml per 100 ml increased both with respect to the control. Rooting was hastened with 0.02 ml extract per 100 ml RM with respect to other concentrations. Moreover, roots produced with 0.02 and 0.2 ml extract per 100 ml RM were longer than with other treatments and in the control. The addition of 0.02 ml extract per 100 ml PM/3 medium, and of 0.02 to 2 ml extract per 100 ml RM improved the photosynthetic activity of cultures after five and four weeks in the proliferation and rooting phases, respectively.

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