Vol. 79 No: 5

Title:
Enhanced shoot and bulblet proliferation of garlic (Allium sativum L.) in bioreactor systems

Authors:
E. K. KIM, E. J. HAHN, H. N. MURTHY and K. Y. PAEK

pp: 818-822

Abstract:
Two types of Balloon Type Bubble Bioreactors (BTBB, air lift type), continuous immersion cultures (with and without raft) and temporary immersion cultures (ebb and flow) were used for mass multiplication of shoots and bulblets of Garlic 'Danyang'. Murashige and Skoog (1962) liquid medium supplemented with 2% sucrose was used for shoot proliferation and shoot proliferation was recorded after three weeks. MS liquid medium supplemented with 11% sucrose and 0.1 mg l^-1 naphthalene acetic acid was used for bulblet induction and growth and bulblet growth was recorded after nine weeks in bioreactor cultures. The continuous immersion types of bioreactors were found suitable for shoot proliferation and 27 shoots were developed from each explant. Biomass of shoots was also highest by this method. Inoculation density of 15 shoots per culture was optimal and aeration of bioreactors with a sparger (0.1 air volume per medium per min) influenced the biomass production. Immersion type (with raft) bioreactor was optimal for bulblet formation. The ratio of inoculum size and media volume affected the growth values. Cold treatment of cultures at 4°C for three weeks enhanced the formation of bulblets. Bulblets were harvested at the end of the culture period, were given a cold treatment of 4°C for eight weeks and transferred to a potting mixture of peat moss and perlite (1:1). Bulblets more than 0.2 g sprouted (90%) within two weeks of transplantation.

Full text: JHSB Subscribers     ISHS members & other users
(PDF 1696323 bytes)

Translate:

Go back to previous page