Vol. 79 No: 6

Title:
Somatic embryogenesis of pistachio from female flowers

Authors:
A. ONAY, V. PIRINÇ, E. TILKAT, Z. AKTÜRK and H. YILDIRIM

pp: 960-964

Abstract:
Induction of somatic embryogenesis from the flower buds of pistachio (Pistacia vera L.) was studied with respect to growth regulators. Sixty-five percent of cultures formed embryogenic calli when placed on modified Murashige and Skoog medium containing 1 mg l^-¹ benzylaminopurine. In contrast, thidiazuron and &alph;-naphthalene acetic acid reduced embryogenesis. Some of the somatic embryos had fused hypocotyls or multiple cotyledons. Abscisic acid was not necessary for maturation. Fewer somatic embryos germinated with 0.5 mg l^-¹ abscisic acid, but more converted into plantlets. Separate, morphologically normal, somatic embryos germinated on semi-solid germination medium, and developed into plantlets. Cytological analysis revealed a chromosome number of 2n = 2x = 30, similar to seedlings. The protocol described in this paper, for the induction of somatic embryos indirectly from female flowers of pistachio, represents a first step towards the production of clonal stocks.

Full text: JHSB Subscribers     ISHS members & other users
(PDF 1675518 bytes)

Translate:

Go back to previous page