Vol. 80 No: 1

Title:
Micropropagation of chestnut rose (Rosa roxburghii Tratt) and assessment of genetic stability in in vitro plants using RAPD and AFLP markers

Authors:
X. P. WEN and X. X. DENG

pp: 54-60

Abstract:
Chestnut rose (Rosa roxburghii Tratt) shows desirable prospects both in fruit exploitation and ornamental plant genetic improvement. A micropropagation system based on axillary bud proliferation was established and optimized for mature trees. Nodal segments, 30-45 d-old, gave the best regeneration after being incubated in half-strength MS medium supplemented with gibberellic acid (GA₃; 2.9 µM), 6-benzyladenine (BA; 2.2 µM), naphthalene acetic acid (NAA; 0.5 µM), and activated charcoal (AC; 300-400 mg l-1). The best multiple-shoot response was obtained with 'Guinong No. 5' and 'white-flower' chestnut rose on a modified MS1 medium supplemented with 2.9 µM GA₃, 2.2-4.4 µM BA, and 0.5 µM NAA. Shoots 4-5 cm in height, cultured for 20-25 d in 1/2 MS medium supplemented with 1.6 µM NAA, 2.5-3.5 µM paclobutrazol, and 300-400 mg l^-1AC showed the best rooting. No polymorphic marker was found among 669 RAPD markers generated with 124 arbitrary primers, in multiplications of 'Guinong No. 5'. To test further the genetic stability of in vitro shoots of 'Guinong No. 5' and 'white-flower' chestnut rose, a total of 1,307 and 1,339 AFLP markers, amplified with 54 selective primer combinations were scored. No aberrant marker profile was observed when in vitro shoots were multiplied through as many as 25 in vitro cycles. These results indicate that this in vitro culture system may provide a useful alternative for chestnut rose multiplication and conservation.

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