Vol. 80 No: 1

Title:
Induction of somatic embryogenesis in Iris hollandica Hort. cv. 'Bronze Queen'

Authors:
F. FIDALGO, A. SANTOS, N. OLIVEIRA, I. SANTOS and R. SALEMA

pp: 135-138

Abstract:
Twin-scale explants of Iris hollandica cv. 'Bronze Queen' produced callus when cultured on a modified Murashige and Skoog medium supplemented with 9.0, 18.1, 27.2 or 36.2 µM 2,4-dichlorophenoxyacetic acid (2,4-D). Induction of embryogenic callus was obtained at 9.0 or 18.1 µM 2,4-D, while higher concentrations of the auxin (27.2 or 36.2 µM) were not effective. Embryo-like structures developed on the surface of embryogenic calli. Somatic embryo maturation was achieved when the bipolar structures produced were cultured on medium without auxin. To improve embryo germination, the developing embryos were transferred to medium supplemented with 0.95 µM abscisic acid (ABA). In this medium, mature embryos developed into normal plantlets. Histological studies confirmed that the structures exhibiting bipolar differentiation were somatic embryos. Regeneration of Iris hollandica cv. 'Bronze Queen' plants through somatic embryogenesis was thus established.

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