Vol. 80 No: 2

Title:
Shoot regeneration and micrografting of micropropagated hybrid tomatoes

Authors:
I. GRIGORIADIS, I. NIANIOU-OBEIDAT and A. S. TSAFTARIS

pp: 183-186

Abstract:
The use of grafted tomato plants is expanding. An in vitro propagation system was established for three commercial F1 cultivars (´Garnet 622`, ´Jumbo` and ´Marvel`) to combine micropropagation with micrografting. Nodal tissue explants were excised from the central stem of plantlets and micropropagated on MS medium. A micropropagation rate of 6 shoots per explant was obtained for all three hybrids. Micropropagated shoots were then micrografted onto 3 week-old ´RT-79` rootstocks with a union rate of over 80%. After acclimatisation, micropropagated plants were established ex vivo, flowered and matured normally. In addition, regeneration protocols were tested using cotyledon explants from 10 d-old seedlings. Adventitious shoot organogenesis from three parts of the cotyledon explants (proximal, middle, distal) was monitored on MS media supplemented with different concentrations of zeatin (Z) and indole-3-acetic acid (IAA). The highest percentage of shoot formation was observed on MS medium supplemented with 0.5 mg l^-1 Z and 0.1 mg l^-1 IAA acid with cotyledon explants that originated from the proximal part. Up to 80% of explants formed adventitious shoots, with an average of three shoots per explant. Three week-old regenerated shoots were placed on MS medium without growth regulators for elongation, rooting and further development of plantlets.

Full text: JHSB Subscribers     ISHS members & other users
(PDF 941810 bytes)

Translate:

Go back to previous page