Vol. 80 No: 4

Title:
Plant regeneration from somatic embryos of interspecific hybrids of grapevine formed in liquid medium

Authors:
V. A. ZLENKO, I. V. KOTIKOV and L. P. TROSHIN

pp: 461-465

Abstract:
Cell suspensions initiated from petiole-derived pro-embryogenic calli gave rise to somatic embryos in three grapevine cultivars, all interspecific hybrids of the genus Vitis. The cultivars differed in the subsequent behaviour of torpedo-stage embryos sub-cultured from liquid onto solid medium. Cultivar ´Bianca` produced shoots on hormone-free medium, while secondary embryos required medium with 0.5 mg l<sup>-1</sup> 6-benzyladenine (BA). In contrast, in cv.´Podarok Magaracha`, shoots were produced on medium with BA, while secondary embryos were formed on hormone-free medium. In the cv.´Intervitis Magaracha`, secondary embryos were formed only on solid medium with 0.5 mg l^-1 indole-3-acetic acid (IAA), and shoot development was observed on media with BA and on hormone-free medium. Secondary embryos were formed intensively on solid medium with 0.5 mg l^-1 IAA in all three cultivars. Plantlets with green cotyledons and hypocotyls which developed from torpedo-stage embryos in liquid medium with 0.5 mg l^-1 BA, and were subsequently cultured on solid, hormone-free medium,converted to callus. Germination of torpedo-stage embryos in liquid medium with 0.5 mg l^-1 gibberelic acid (GA3) for 5 d vs. 10 d incubation led to better subsequent shoot production from the developed plantlets with green cotyledons and hypocotyls after sub-culturing them onto solid medium with 0.5 mg l^-1 BA in all three grapevine cultivars.

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