Vol. 80 No: 5

Title:
Plant regeneration from immature zygotic embryos of chestnut rose (Rosa roxburghii Tratt) through organogenesis

Authors:
X.P. WEN and X.X. DENG

pp: 638-642

Abstract:
An efficient method for plantlet regeneration through organogenesis from immature zygotic embryos of chestnut rose (Rosa roxburghii Tratt) has been developed. Immature 35-55 d-old cotyledonary zygotic embryos of cv. ´Guinong No. 5` and White-Flower chestnut rose (R. roxburghii Tratt f. candida) were first inoculated onto solid medium containing half-strength Murashige and Skoog macro-elements (1/2 MS), 2.5 µM indole-3-acetic acid (IAA), 4.5 µM 6-benzyladenine (BA), 500 mg l^-1 lactalbumin hydrolysate (LH) and 0.7% (w/v) agar, in the dark for 15 d. Embryos were then transferred to solid media supplemented with different concentrations of various cytokinins, in combination with other plant growth regulators (PGRs), to induce shoot regeneration. Modified MS medium (MS1: 1/2 strength NH4NO3; other components at full-strength) supplemented with 2.5 µM IAA, 4.5 µM BA, 3.0 µM gibberellic acid (GA) and 500 mg l^-1 LH was best for shoot regeneration. Prior to root induction, shoots were transferred onto solid MS1 medium containing 2.2 µM BA, 3.0 µM GA and 0.5 µM -naphthalene acetic acid (NAA) for elongation. Rooting efficiency varied depending upon the auxin present in the medium.The highest rooting percentage (93%) was attained on 1/2 MS medium with 1.5 µM NAA, 2.5-3.5 µM paclobutrazol (PP333) and 300 mg l^-1 activated charcoal (AC). Shoots (4-5 cm high) formed several adventitious roots 20-25 d after culture on rooting medium. After acclimatisation for 10-15 d in autoclaved soil, plants showed 100% survival after transfer to the field

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