Vol. 81 No: 3

Title:
Genetic relationships among sea buckthorn varieties from China, Russia and Mongolia using AFLP markers

Author:
C.J. RUANA

pp: 409-414

Abstract:
Sea buckthorn (Hippophae rhamnoides) is important environmentally, commercially and as a new berry crop. AFLP markers were applied to evaluate genetic relationships among 15 selected sea buckthorn varieties from China, Russia and Mongolia. Nine primer combinations generated 545 AFLP bands, 451 (83%) of which were polymorphic. Genetic similarities (Dice coefficients) ranged from 0.545 - 0.917, with a mean of 0.749. Nei`s genetic distances ranged from 0.086 - 0.601, with a mean of 0.295. Cluster analysis identified ´Zhongguoshaji` (H. rhamnoides ssp. sinensis) from China as the most distantly related to the 14 other varieties. At a Dice coefficient of 0.770, nine varieties from Russia, one from Mongolia (H. rhamnoides ssp. mongolica) and two hybrids from China were grouped into two main clusters. The three remaining varieties (´Zhongguoshaji`, ´Zajiaoliangzhong` and ´Zhongguoyou`) from China were not grouped at the Dice coefficient of 0.770. Cluster I included ´Huoguo`, ´Zeliang`, ´Liaohuyihao`, ´Chengse`, ´Huoguang`, ´Wulangemu`, ´Xiangyang`, ´Chuyi` and ´Liaohuerhao`. Cluster II consisted of ´Nuyou`, ´Hongyun` and ´Alniyi`. These data provide a more rigorous scientific basis for the identification of sea buckthorn varieties, the collection of germplasm and the development of crossing strategies in a breeding programme.

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