The Journal of Horticultural Science & Biotechnology
Vol. 81 No: 3
Development of a medium for in vitro culture of Galanthus species based on the mineral composition of bulbs
I. STAIKIDOU, C. SELBY and G.R. HANKS
To optimise conditions for micropropagating Galanthus species, a basal medium (G) was developed based on mineral analyses of G. nivalis, G. nivalis ´Flore Pleno` and G. elwesii bulbs. Compared with Murashige and Skoog (MS) medium, the main features of G medium were increased concentrations of Cu (× 30.4), P (× 3.6), Ca (× 1.9), Mg (× 1.3) and S (× 1.2) and reduced levels of Mn (× 0.07), Zn (× 0.59) and K (× 0.65). The efficacy of G medium in supporting bulblet initiation on bulb chip e×plants, bulblet multiplication (on media supplemented with 30 g l-1 sucrose, 1.0 mg l-1 6-benzylaminopurine and 0.1 mg l-1 naphthalene acetic acid), and bulblet growth (on plant growth regulator-free media with 60 g l-1 sucrose and 5 g l-1 activated charcoal) was compared with MS medium over a range of dilutions (full-, 1/2-, 1/-4-, and 1/8-strength). Bulblet initiation was superior on G medium for G. nivalis and G. nivalis ´Flore Pleno`, but inferior for G. elwesii.The choice of basal medium did not influence bulblet multiplication, although multiplication was reduced on both media diluted to 1/8-strength. G medium supported bulblet growth and rooting better than MS medium, while dilution of either medium reduced bulblet growth and rooting. Using G medium in place of MS medium during bulblet multiplication greatly reduced hyperhydration with G. elwesii, as did dilution of either of the basal media.
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