The Journal of Horticultural Science & Biotechnology
Vol. 81 No: 3
Zeatin overcomes thidiazuron-induced inhibition of shoot elongation and promotes rooting in strawberry culture in vitro
SAMIR C. DEBNATH
The effects of 0, 2, 4 or 8 μM thidiazuron (TDZ) and explant type on adventitious shoots regenerated on excised leaf disks, sepals (calyx) and petiole halves of greenhouse-grown ŽBounty` strawberry (Fragaria × ananassa Duch.) were studied. A moderate concentration (2 - 4 μM TDZ) supported bud and shoot regeneration without an intermediary callus phase on young expanding sepals, or on leaf disks with the adaxial side touching the culture medium, or on petiole halves placed horizontally with their cut-sides in contact with the medium, all maintained for 14 d in the dark. Sepals proved more effective than leaf discs and petiole segments for regenerating shoots. Another experiment compared the effects of 0, 0.5, 1, 2 and 4 μM TDZ or zeatin on shoot proliferation and rooting of sepal-derived adventitious shoots. Media containing TDZ generally promoted more callus formation and suppressed elongation and rooting of shoots. Shoots proliferated and roots developed best when explants were cultured in medium supplemented with 1 or 2 μM zeatin. An in vitro cloning protocol that enables shoot proliferation and rooting of strawberry explants in the same medium is expected to be applied for commercial micropropagation.
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