Vol. 81 No: 4

Title:
Somatic embryogenesis and plantlet regeneration from encapsulated embryos of Selinum tenuifolium

Authors:
MEENA JOSHI, UPPEANDRA DHAR and SUMIT MANJKHOLA

pp: 570-574

Abstract:
This paper reports, for the first time, somatic embryogenesis and synthetic seed production in Selinum tenuifolium Wall. Mature leaf explants inoculated in Murishige and Skoog (MS) medium supplemented with 3 μM 2,4-dichlorophenoxyacetic acid (2,4-D), containing 3% (w/v) sucrose and 0.7% (w/v) agar, induced 67% callus. Maximum production of globular structures, their differentiation into embryos and germination, occurred with a combination of 2 3 μM benzyladenine (BA) and 2 μM indole-3-butyric acid (IBA). To protect somatic embryos and produce synthetic seeds, gel capsules were standardised using a combination of sodium alginate and calcium nitrate concentrations. Gel capsules were most effective when formed with a combination of 3% (w/v) sodium alginate and 100 mM calcium nitrate for 30 min. The addition of MS medium to alginate capsules with 3% (w/v) sodium alginate, 3% (w/v) sucrose, 2 3 μM BA and 2 3 μM IBA significantly improved their germination rate to 77.8%, as well as their resulting shoot length (5.6 cm) and root length (7.2 cm), compared to controls (57.8%). Most plantlets (66%) survived under nursery condition. Storage at 4°C for different periods (10 d or 20 d) significantly (P 〈 0.05) reduced the percentage survival and germination of somatic embryos and artificial seeds compared to controls or 5 d storage.

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